Expression of Gsdf. (A) Real-big date PCR off Gsdf in XX and you can XY fry out-of 2 weeks before hatching (dbh) in order to ten months shortly after hatching (dah). Bluish bars, expression off PG5 for the Y chromosome (Gsdf Y ); red, the latest X chromosome (Gsdf X ). Significant differences was analyzed that with a couple of-method research from difference (ANOVA) followed by Bonferroni post-screening. Columns and you can mistake taverns represent imply ± SEM (n = 4 each developmental stage). (b–d) Into the situ hybridization regarding Gsdf regarding gonad. (B) Term out-of Gsdf when you look at the 5-dah XY fry. Gsdf are seen simply into the somatic cells nearby germ cells. (C) Phrase out of Gsdf in 5-dah XX fry. Continue reading